Multiple Human Tyrosine Hydroxylase Enzymes, Generated Through Alternative Splicing, Have Different Specific Activities in Xenopus Oocytes
- 1 August 1988
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 51 (2) , 652-655
- https://doi.org/10.1111/j.1471-4159.1988.tb01088.x
Abstract
A single human tyrosine hydroxylase (HTH) gene has been shown previously to generate four species of mRNA by alternative splicing. The four different HTH mRNAs were independently synthesized in vitro, using the SP6 transcription system. Each of these mRNA species was able to direct the synthesis of an active form of TH following injection into Xenopus oocytes. Quantitation of synthesized HTH polypeptides allowed the determination of the relative specific activity of each individual HTH form. A significant difference in specific activity was found between each form, suggesting that alternative splicing may play a role in regulating HTH activity in vivo.Keywords
This publication has 21 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- Predicted Amino Acid Sequence of Bovine Tyrosine Hydroxylase and Its Similarity to Tyrosine Hydroxylases from Other SpeciesJournal of Neurochemistry, 1988
- Cloning of Quail Tyrosine Hydroxylase: Amino Acid Homology with Other Hydroxylases Discloses Functional DomainsJournal of Neurochemistry, 1987
- Isolation of a novel cDNA clone for human tyrosine hydroxylase: Alternative RNA splicing produces four kinds of mRNA from a single geneBiochemical and Biophysical Research Communications, 1987
- ALTERNATIVE SPLICING: A UBIQUITOUS MECHANISM FOR THE GENERATION OF MULTIPLE PROTEIN ISOFORMS FROM SINGLE GENESAnnual Review of Biochemistry, 1987
- Alternative Splicing: A Ubiquitous Mechanism For The Generation Of Multiple Protein Isoforms From Single GenesAnnual Review of Biochemistry, 1987
- Two forms of the la antigen-associated invariant chain result from alternative initiations at two in-phase AUGsCell, 1986
- A single RNA species injected in Xenopus oocyte directs the synthesis of active tyrosine hydroxylaseFEBS Letters, 1986
- Cell-free synthesis and membrane insertion of mouse H-2Dd histocompatibility antigen and β2-microglobulinCell, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970