A reliable method for assessing topographical arrangement of proteins in the chromaffin granule membrane
- 1 January 1985
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 10 (1) , 19-32
- https://doi.org/10.1007/bf00964769
Abstract
An efficient and reliable method for preparing chromaffin granules and membranes for topographical analysis has been designed to meet the following criteria. Only labeling of truly intact organelles is analyzed for cytoplasmic surface probes. No. resealing of open membranes occurs under specified reaction conditions. Saturation of available sites must be demonstrated. Problems in meeting these conditions are discussed, in particular the interference of granule lysate with labeling reactions.This publication has 15 references indexed in Scilit:
- The 97-kd α-actinin-like protein in chromaffin granule membranes from adrenal medulla: evidence for localization on the cytoplasmic surface and for binding to actin filamentsNeuroscience, 1983
- NADH: (acceptor) oxidoreductase from bovine adrenal medulla chromaffin granulesArchives of Biochemistry and Biophysics, 1982
- Th molecular organization of adrenal chromaffin granulesNeuroscience, 1980
- Organisation of the proteins of the chromaffin granule membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1979
- Transport of catecholamines by resealed chromaffin-granule ‘ghosts’Biochemical Journal, 1974
- [39] Adrenal chromaffin granules: Isolation and disassemblyPublished by Elsevier ,1974
- Membrane proteins of chromaffin granules. Dopamine β-hydroxylase, a major constituentBiochemical Journal, 1972
- The effect of salts on catecholamine fluxes and adenosine triphosphatase activity in storage vesicles from the adrenal medullaBiochemical Journal, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The arrangement of proteins in the human erythrocyte membraneBiochemical and Biophysical Research Communications, 1970