Plant Microbody Proteins, II. Purification and Characterization of the Major Protein Component (SP-63) of Peroxisome Membranes

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Abstract
The major component of membranes of microbodies from green leaves of Lens culinaris was a protein of a subunit MW of 63,000. This protein, referred to as SP-63, seemed to be unique to microbodies and could not be detected when plastids or mitochondria were analyzed. It is probably a structural protein and was not solubilized by cholate, Triton X-100, chloroform/methanol, or 0.2 M KCl. Solubilization from purified membranes was achieved with guanidinium chloride or sodium dodecylsulfate. The protein was separated from minor contaminating components by chromatography on Sepharose 4B or Sephadex G-150 employing 0.1% sodium dodecylsulfate or 4 M urea as eluent. It was homogeneous upon sodium dodecylsulfate gel electrophoresis and did not give a positive glycoprotein stain.

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