Studies on epstein-barr virus-related antigens. II. Biochemical properties of soluble antigen in raji burkitt lymphoma cells

Publisher Website
Google Scholar
Abstract
Biochemical properties of Epstein‐Barr virus (EBV)‐related soluble antigen in non‐producer Raji Burkitt lymphoblastoid cells, assayed by the indirect single radial immunodiffusion (ISRD) test, were investigated. The soluble ISRD antigen retained activity even after exposure to a temperature of 80°C. The antigen was precipitated in 40% saturated ammonium sulfate and ISRD activity was recovered from the precipitate when reconstituted into a solution. Isoelectric focusing and crossed immuno‐electrophoretic analyses revealed that the isoelectric point of the ISRD antigen is pH 4.8 with an electrophoretic mobility similar to that of serum α‐globulin. With Sephadex G‐200 or Sepharose 6B gel filtration, ISRD activity was obtained as a single peak which corresponded to the activity absorbing anti‐complement immunofluorescence. The molecular weight of the present EBV‐related soluble antigen was estimated to be 220,000–240,000 daltons.