Micromethod for the Estimation of Plasma Fibrinogen
- 1 January 1965
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 11 (1) , 10-16
- https://doi.org/10.1093/clinchem/11.1.10
Abstract
Fibrinogen is quantitatively precipitated from plasma by 1.20 M phosphate buffer. The amount of precipitated fibrinogen is determined turbidimetrically at 450 mµ. The method requires 0.25 ml. of plasma for the blank and sample. Reliability of the method has been established by comparison with an ammonium sulfate turbidimetric method and a colorimetric method, demonstration of specificity, variation in incubation temperature, demonstration of optimal molarity and pH, and replicate analyses.Keywords
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