Wheat endopeptidase: genetic control, polymorphism, intrachromosomal gene location, and alien variation

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Abstract
Isoelectric focusing separations of aqueous extracts of mature embryo tissue of 'Chinese Spring' wheat (Triticum aestivum) confirmed the previous gene location of the endopeptidase-1 (Ep-1) homoeoallelic series to the long arms of the group 7 chromosomes. Screening of a range of wheat varieties revealed a significant degree of polymorphism at each of the three loci, Ep-A1, Ep-B1, and Ep-D1, including three alleles at Ep-A1, five at Ep-B1, and three at Ep-D1. One allele at each locus was null. Polymorphism at Ep-B1 allowed this locus to be mapped both with respect to a number of other markers on 7BL and from the breakpoint of the 5BL.7BL translocation. A close linkage with the leaf rust resistance gene Lr14a was established and the locus was independent of the centromere. A number of alien relatives of wheat were found to carry distinct homoeoalleles on their group 7 chromosomes.Key words: wheat, endopeptidase, isozymes, isoelectric focusing.