Gas Chromatographic Analysis of Cyclophosphamide in Plasma and Tissues Using Nitrogen-Phosphorus Detection
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 12 (8) , 905-915
- https://doi.org/10.1080/00032717908059770
Abstract
A gas chromatographic method for the analysis of cyclophosphamide in plasma, blood, and organ tissues is described. This method involves extraction of aliquots of plasma or tissue homo-genate in alkaline condition with ether. The extracted drug is derivatized with heptafluorobutyric anhydride followed by gas chromatographic separation via a glass column of 183 cm × 2 mm i. d. packed with 3% SE-30 on chromosorb W-HP. The derivatized cyclophosphamide and isophosphamide, an added internal standard, are detected by a nitrogen-phosphorus detector. The sensitivity limit of this method is 10 ng per gm or ml of sample and gives linearity over 100-fold of concentration range.Keywords
This publication has 3 references indexed in Scilit:
- Simple and sensitive method for the determination of cyclophosphamide by means of a nitrogen—phosphorus-selective detectorJournal of Chromatography B: Biomedical Sciences and Applications, 1978
- Quantitative GLC Determination of Cyclophosphamide and Isophosphamide in Biological SpecimensJournal of Pharmaceutical Sciences, 1974
- Mass spectrometric study of the distribution of cyclophosphamide in humansToxicology and Applied Pharmacology, 1973