LYSOZYME AND ALBUMIN RADIOIMMUNOASSAYS - NEW TECHNIQUES FOR THE STUDY OF PROTEINURIA

  • 1 January 1980
    • journal article
    • research article
    • Vol. 17  (6) , 526-528
Abstract
To facilitate the study of mechanisms and patterns of proteinuria, radioimmunoassays [RIA] for human lysozyme (LZM) and albumin (alb) were established to permit quantitation of physiologic amounts of these proteins in urine. Commercially available LZM and alb preparations were radiolabled with I125, and single antibody, competitive protein binding assays were developed. Separation of free and antibody-bound radioprotein was achieved with 20% polyethylene glycol. LZM and alb 24 h excretion rates for 12 normal subjects were 7 to 64 .mu.g and 2.3 to 16.1 mg, respectively. Of 6 renal disease patients with undetectable urine LZM by bioassay, 5 had elevated LZM concentrations by RIA. The ease of establishing and performing these assays and their reproducibility suggest that they may have clinical and investigative value.

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