Studies on a Radioassay for Intrinsic Factor Antibody: Comparison of Methods and False Positive Results due to Elevated Serum B12 Levels
Open Access
- 1 September 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 82 (3) , 300-304
- https://doi.org/10.1093/ajcp/82.3.300
Abstract
Availability of a kit method (Corning Immo Phase® IFAB) for intrinsic factor antibody (IFAB) has made it possible for a routine radioimmunoassay (RIA) laboratory to test for the antibody, thereby providing another aid in diagnosing pernicious anemia. Comparison of data from a charcoal method with the kit method was favorable, each method detecting 35 (74%) positives and 12 (26%) negatives of 47 pernicious anemia patients. Compared with a charcoal method study the kit method had fewer false positives due to elevated serum B12. False positive results occurred for only 24 hours after a 1-mg injection of B12, and results remained negative the following seven days. The authors’ studies supported the manufacturer’s statement that results are unreliable when the serum B12 level exceeds 3,500 pg/ml. Clinical experience with the Corning Immo Phase IFAB test and false positive results is summarized.Keywords
This publication has 3 references indexed in Scilit:
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- Rapid Charcoal Assay for Intrinsic Factor (IF), Gastric Juice Unsaturated B12 Binding Capacity, Antibody to IF, and Serum Unsaturated B12 Binding CapacityBlood, 1965
- ANTIBODY TO INTRINSIC FACTOR IN SERUM FROM PATIENTS WITH PERNICIOUS ANEMIA*Journal of Clinical Investigation, 1962