Intramitochondrial localization of glutathione-peroxidase (GSH-PO) in the rat adrenocortical cells.
- 1 January 1984
- journal article
- research article
- Published by Japan Society of Histochemistry & Cytochemistry in ACTA HISTOCHEMICA ET CYTOCHEMICA
- Vol. 17 (2) , 127-137
- https://doi.org/10.1267/ahc.17.127
Abstract
Immunocytochemical localization of glutathione-peroxidase (GSH-PO) in the rat adrenocortical cells was observed not only in cytosol (C-GSH-PO) but in mitochondria (M-GSH-PO) by the direct peroxidase labeled antibody method using anti-GSH-PO IgG Fab'' fragment. In the untreated rat adrenocortical cells, C-GSH-PO was observed mainly in the outer part of the zona fasciculata and zona reticularis. M-GSH-PO was observed mainly in the inner part of zona fasciculata. Biochemical GSH-PO activity in the cytosol and mitochondrial fractions obtained from whole adrenal gland was comparable to the immunocytochemical results. In the hypophysectomized rat adrenocortical cells, markedly atrophic inner part of zona fasciculata showed only C-GSH-PO, and biochemical activity of the cytosol GSH-PO was markedly increased. This atrophic zone contains accumulated cholesterol esters. By ACTH administration to the hypophysectomized rats, markedly hypertrophic outer part of zona fasciculata showed increased immunocytochemical localization of M-GSH-PO. And biochemically, mitochondrial GSH-PO activity in this group was higher than that of the hypophysectomized group. Evidently, the C-GSH-PO has its role in the reduction of cholesterol ester hydroperoxides and the MI-GSH-PO in the reduction of sterol hydroperoxides produced during steroidogenesis.This publication has 0 references indexed in Scilit: