Novel avidin and streptavidin binding sequences found in synthetic peptide libraries

Abstract
Synthetic resin‐bound peptide libraries made of protein l‐amino acids have been synthesized. During screening of the libraries, peptides that bind to avidin have been identified containing a novel motif with two histidines separated by one residue. A sub‐library was synthesized and screened, and new critical residues appeared surrounding the two histidines. Additionally peptide libraries made of d‐amino acids have been screened with avidin and streptavidin and novel motifs have been found.