NEW CULTURE MEDIUM FOR GROWTH AND SPORULATION OF ENTOMOPHTHORALES SPECIES, PATHOGENS OF APHIDS

  • 1 January 1978
    • journal article
    • research article
    • Vol. B129  (3) , 463-476
Abstract
None of the 3 specific egg yolk fractions (neutral lipids, polar lipids and proteins) was necessary for growth and sporulation of species including Entomophthora aphidis, E. phalloides and E. thaxteriana, normally grown on egg yolk media. Egg yolk could be replaced by a sunflower oil-yeast extract mixture. E. thaxteriana was especially well-examined. Various animal and vegetable fats and even chemically pure triglycerides could replace sunflower oil as a C source. Yeast extract was the best N source tested. In liquid culture, sporulation was directly dependent on yeast extract concentration. When yeast extract concentration was held constant, an increase in oil concentration did not produce an increase in sporulation. High dextrose concentrations inhibited resting spore formation. Liquid cultures of E. thaxteriana regularly produced 3 .times. 106 azygospores/ml in a 20 l fermentor.

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