Interleukin‐10 inhibits the primary allogeneic T cell response to human epidermal Langerhans cells

Publisher Website
Google Scholar
Abstract
In this study, we analyzed the effect of interleukin‐10 (IL‐10) on the primary allogeneic T cell response induced by human Langerhans cells (LC), the dendritic cells from epidermis. We showed that IL‐10 strongly inhibited the T cell response, provided it was added at the beginning of the mixed epidermal cell lymphocyte reaction (MELR). Proliferation of both CD4+ and CD8+ T cell subsets was affected by the cytokine. An inhibitory effect of IL‐10 on human LC allostimulatory function was evidenced by the fact that IL‐10‐preincubated LC, but not IL‐10‐preincubated T cells, can display inhibitory effect. LC treatment with IL‐10 partially inhibited the increase of HLA‐DR expression on cultured LC as the percentage of highly positive HLA‐DR cells was lower than that observed in the absence of the cytokine. IL‐10 inhibited T cell alloreaction induced by 2‐day‐cultured human LC which constitutively display high levels of HLA class II, as well as ICAM‐1 and LFA‐3 antigens. This suggests that the suppressive effect of the cytokine was not merely related to an impaired up‐regulation of these molecules. Addition of IL‐1 during the MELR potentiated the allogeneic T cell proliferation and could reverse, at least partly, the inhibitory effect of IL‐10. Collectively, these data indicate that IL‐10 can prevent the alloreaction induced by human dendritic cells, providing new insights into the potential clinical use of this cytokine.

This publication has 40 references indexed in Scilit: