Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004
Open Access
- 1 May 2006
- journal article
- research article
- Published by FapUNIFESP (SciELO) in Memórias do Instituto Oswaldo Cruz
- Vol. 101 (3) , 291-294
- https://doi.org/10.1590/s0074-02762006000300012
Abstract
Rotavirus is a major cause of infantile acute diarrhea, causing about 440,000 deaths per year, mainly in developing countries. The World Health Organization has been recommending the assessment of rotavirus burden and strain characterization as part of the strategies of immunization programs against this pathogen. In this context, a prospective study was made on a sample of 134 children with acute diarrhea and severe dehydration admitted to venous fluid therapy in two state hospitals in Rio de Janeiro, Brazil, from February to September 2004. Rotavirus where detected by polyacrylamide gel electrophoresis (PAGE) and by an enzyme-linked immunoassay to rotavirus and adenovirus (EIARA) in 48% of the children. Positive samples for group A rotavirus (n = 65) were analyzed by reverse transcription/heminested multiplex polymerase chain reaction to determine the frequency of G and [P] genotypes and, from these, 64 samples could be typed. The most frequent G genotype was G1 (58%) followed by G9 (40%). One mixed infection (G1/G9) was detected. The only [P] genotype identified was [8]. In order to estimate the rotavirus infection frequency in children who acquired diarrhea as hospital infection in those hospitals, we studied 24 patients, detecting the pathogen in 41% of them. This data suggest that genotype G9 is an important genotype in Rio de Janeiro, with implications to the future strategies of vaccination against rotavirus, reinforcing the need of continuous monitoring of circulating strains of the pathogen, in a surveillance context.Keywords
This publication has 19 references indexed in Scilit:
- Sentinel Hospital Surveillance for Rotavirus Diarrhea in Taiwan, 2001–2003The Journal of Infectious Diseases, 2005
- Epidemiology and Burden of Rotavirus Diarrhea in Thailand: Results of Sentinel SurveillanceThe Journal of Infectious Diseases, 2005
- Rotavirus vaccines and the prevention of hospital-acquired diarrhea in childrenVaccine, 2004
- A Rotavirus Vaccine for Prophylaxis of Infants Against Rotavirus GastroenteritisThe Pediatric Infectious Disease Journal, 2004
- Rotavirus A infections and reinfections: genotyping and vaccine implicationsJornal de Pediatria, 2004
- Safety, efficacy, and immunogenicity of a live, quadrivalent human-bovine reassortant rotavirus vaccine in healthy infantsThe Journal of Pediatrics, 2004
- Changing Patterns of Rotavirus Genotypes in Ghana: Emergence of Human Rotavirus G9 as a Major Cause of Diarrhea in ChildrenJournal of Clinical Microbiology, 2003
- Rotavirus Genotypes P[4]G9, P[6]G9, and P[8]G9 in Hospitalized Children with Acute Gastroenteritis in Rio de Janeiro, BrazilJournal of Clinical Microbiology, 2001
- Rotavirus G and P types circulating in Brazil: characterization by RT-PCR, probe hybridization, and sequence analysisArchiv für die gesamte Virusforschung, 1996
- Efficacy Of A Quadrivalent Rhesus Rotavirus-Based Human Rotavirus Vaccine Aimed At Preventing Severe Rotavirus Diarrhea In Infants And Young ChildrenThe Journal of Infectious Diseases, 1996