Specific reversal of cytolytic T lymphocyte – target cell interaction

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Abstract
A functional assay is described that measures the reversal of specific cytolytic T cell (CTL)‐target cell binding. Binding of 51Cr‐labeled P815 cells was stable in suspension but could be readily reversed by the addition of unlabeled P815 cells. The reversal of CTL‐tumor cell and CTL‐spleen cell binding was H‐2 specific; only cells of the same H‐2 type as the bound target cell could induce reversal. In all cases, tumor cells were substantially more efficient than spleen cells in inducing specific reversal.