Functional expression of Ca2+ ‐mobilizing α‐thrombin receptors in mRNA‐injected Xenopus oocytes
- 26 March 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 262 (2) , 330-334
- https://doi.org/10.1016/0014-5793(90)80221-4
Abstract
α‐Thrombin (TH) initiates a program of intracellular events that lead to DNA replication in quiescent CCL39 Chinese hamster lung fibroblasts via membrane receptors that have yet to be characterized at a molecular level. Functional TH receptors were expressed in Xenopus laevis oocytes following injection of poly(A)+ RNA from TH‐responsive CCL39 cells; their presence was demonstrated by TH‐stimulated 45Ca2+ efflux or Ca2+dependent Cl− channel activation. In voltage clamp experiments on microinjected oocytes a Ca2+ ‐activated Cl− current was detected in response to TH (0.2–10 ). The TH response was blocked by a specific TH inhibitor, and potentiated by addition of FGF or intracellular injection of GTP‐γ‐S.Keywords
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