MONOCLONAL-ANTIBODY LOCALIZATION OF LEWIS ANTIGENS IN FIXED TISSUE

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 50  (4) , 394-400
Abstract
Monoclonal antibodies that bind specifically with Lewisa (Lea) and Lewisb (Leb) antigens were used in an immunoperoxidase assay to characterize Lewis (Le) antigenic profile of a variety of fixed tissues. Representative sections of normal and malignant [human] tissue from the stomach, colon, pancreas and kidney were examined and compared. Lea antigen was expressed more often in gastric adenocarcinomas (80%) than normal gastric mucosa (40%). In gastric tumors with concomitant expression of Le antigens, larger areas within an individual tumor expressed Lea antigen rather than Leb antigen. Expression of Leb antigen in normal colonic tissue was seen only in the proximal colon and not distal colon. Colon carcinomas expressed Leb antigen (64%) regardless of where the primary arose. Leb antigen was expressed in large pancreatic ducts (3 of 3) more often than Lea antigen (1 of 3); exclusive expression of Lea antigen was demonstrated in the proximal convoluted tubules of normal kidney (3 of 3) and in renal cell carcinomas (6 of 8).