Formation of covalent circles of lambda DNA by E. coli extracts.
- 1 January 1967
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 57 (1) , 148-155
- https://doi.org/10.1073/pnas.57.1.148
Abstract
Extracts of Escherichia coli convert [lambda] DNA to covalent circles. The reaction requires Mg ion and prior formation of H-bonded circles of the [lambda] DNA. Extracts of -sensitive, [lambda] -lysogenic and induced [lambda]-lysogenic strains and extracts of 2 recombination-deficient mutants were active. The covalent circles appear by sedimentation to be less twisted than those made intracellularly. Their sedimentation rate is sharply increased by addition of actinomycin.This publication has 20 references indexed in Scilit:
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