Coexistence of Cardiac-Type and Fast Skeletal-Type Myosin Light Chains in Embryonic Chicken Cardiac Muscle1
- 1 July 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 94 (3) , 1025-1028
- https://doi.org/10.1093/oxfordjournals.jbchem.a134401
Abstract
Myosin from embryonic chicken ventricle contained a light chain component which comigrated with fast skeletal myosin light chain 1 (Lf1) on two dimensional electrophoresis in addition to cardiac type light chains (Lc1 and Lc2. Immunoblot analysis showed that this minor light chain band reacted with anti-Lf1 antibody. Antigens binding with anti-Lc1, and anti-Lf1 antibodies were located on myofibrils in embryonic cardiac muscle cells in vivo and in vitro. From these observations, we conclude that a small amount of Lf1 exists in embryonic chicken cardiac muscle.Keywords
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