MONOCLONAL-ANTIBODIES TO BRUCELLA SURFACE-ANTIGENS ASSOCIATED WITH THE SMOOTH LIPOPOLYSACCHARIDE COMPLEX

  • 1 January 1984
    • journal article
    • research article
    • Vol. 45  (5) , 967-971
Abstract
Hybridomas producing antibodies to determinants associated with the lipopolysaccharide (LPS) of B. abortus and B. melitensis were obtained by polyethylene glycol fusion of the SP2/0 [mouse] myeloma cell line with B lymphocytes harvested from a Sprague-Dawley-derived rat previously immunized with whole B. abortus strain 1119 organisms. Two clones, BRU38 and BRU28, were selected for their ability to react with whole B. abortus organisms and purified smooth LPS (f5p). The BRU38 monoclonal antibodies were absorbed with live, rough strain 45/20 and smooth strains of B. abortus and B. melitensis organisms; only smooth strains absorbed the antibody activity from BRU28. Complete inhibition of the monoclonal''s activity was achieved with crude smooth LPS, a purified f5p fraction and a H2O-soluble acids degraded polysaccharide. Absorption of BRU38 and BRU28 with rough Brucella LPS, polysaccharide B antigen, keto-deoxyoctanoic acid or with several sugars and fatty acids known to be components of the Brucella LPS complex had no effect on the monoclonals. Thus, antigenic determinants are associated with the smooth LPS complex, probably with the O side chain, and are expressed patchwise and in different quantities on several strains of B. abortus and B. melitensis. The B. abortus rough strain 45/20 contains surface determinants which lead to the agglutination of smooth strain 1119 organisms. The potential use of monoclonals in competitive enzyme-linked immunosorbent assays for diagnostic purposes is discussed.

This publication has 0 references indexed in Scilit: