A cAMP-dependent protein kinase is present in differentiating Dictyostelium discoideum cells.
- 1 January 1982
- journal article
- Vol. 1 (9) , 1063-8
Abstract
We demonstrate the occurrence of a cAMP-dependent protein kinase in Dictyostelium discoideum cells at the terminal stage of differentiation. A cAMP-binding component was purified to homogeneity by affinity chromatography. This subunit inhibits the activity of purified catalytic subunit from beef heart protein kinase; the inhibition is reversed upon addition of cAMP. The protein is highly specific for cAMP and has a dissociation constant of 4 nM. The isolated regulatory subunit is a monomer of 39 K, with a sedimentation coefficient of 3.5S and a frictional coefficient of 1.24. The differences between this regulatory subunit and regulatory subunits of protein kinases from other sources are discussed.Keywords
This publication has 36 references indexed in Scilit:
- Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductasesPublished by Elsevier ,2003
- Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivityAnalytical Biochemistry, 1981
- Aggregation in Dictyostelium discoideumCell, 1981
- A cAMP-binding protein from Dictyostelium discoideum regulates mammalian protein kinaseBiochemical and Biophysical Research Communications, 1981
- Two different intrachain cAMP binding sites of cAMP-dependent protein kinases.Journal of Biological Chemistry, 1980
- Adenosine-3': 5'-Monophosphate-Binding Proteins from Bovine Kidney. Isolation by Affinity Chromatography and Limited Proteolysis of the Regulatory Subunit of Protein Kinase IIEuropean Journal of Biochemistry, 1978
- Developmentally regulated cyclic AMP-dependent protein kinases in dictyostelium discoideumCell, 1977
- Chemotactic signals induce cell differentiation in Dictyostelium discoideum.Proceedings of the National Academy of Sciences, 1975
- Control of cell-contact sites by cyclic AMP pulses in differentiating Dictyostelium cellsNature, 1975
- Physical Properties of a Purified Cyclic Adenosine 3′:5′-Monophosphate-dependent Protein Kinase from Bovine Heart MuscleJournal of Biological Chemistry, 1973