Rapid histamine assays: a method and some theoretical considerations

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Abstract
1 An automatic method for doing assays on superfused isolated tissues has been developed using the Autoanalyser sample turntable. 2 Responses in the range used for assays are independent of the preceding responses when elicited at 1 min intervals. A longer interval was found to be necessary when using the conventional immersion method. 3 The index of precision was similar to that reported for other superfusion methods. 4 The assay method behaved as though it were assaying histamine selectively in two samples of material released by the anaphylactic reaction of passively sensitized guinea-pig lung. Responses to anaphylactically released material were also independent of the preceding response at 1 min intervals. 5 Some theoretical considerations are discussed concerning the arrangement of doses, and the interpolation of results, in 2 + 1 dose assays. The object was to maximize speed while still allowing a valid estimate of the potency and its error.

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