RAPID AND REVERSIBLE INHIBITION OF JUNCTIONAL COMMUNICATION BY TUMOR PROMOTERS IN A MOUSE-CELL LINE
- 1 January 1983
- journal article
- research article
- Vol. 43 (8) , 3614-3618
Abstract
Mouse epidermal HEL-37 cells rapidly transferred microinjected fluorescein between contacting cells. This transfer was strongly inhibited by tumor-promoting phorbol esters and mezerein but not by non-promoting derivatives. The onset of the inhibition occurred after 5-10 min of exposure to the test compound and was complete within 20 min. Using a wash-off procedure, inhibition of dye transfer by phorbol-12,13-dibutyrate was fully reversed by 100 min, after a lag of 40-60 min. The recovery of dye transfer competence was blocked by puromycin. Both 12-O-tetradecanoylphorbol-13-acetate and monensin caused elevated Na+ levels in HEL-37 cells, but the latter compound did not inhibit dye transfer. The promoter inhibition of transfer was not a consequence of a Na+-induced increase in cytoplasmic Ca2+ concentration.This publication has 24 references indexed in Scilit:
- Inhibition of intercellular communication between liver cells by the liver tumor promoter 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethaneCancer Letters, 1981
- Tumor promoter-induced changes in the permeability of epithelial cell tight junctionsCell, 1981
- Tumor promoter inhibition of intercellular communication between cultured mammalian cellsCarcinogenesis: Integrative Cancer Research, 1981
- Dispersal of junctional particles, not internalization, during the in vivo disappearance of gap junctionsCell, 1980
- INHIBITION OF METABOLIC COOPERATION IN CHINESE-HAMSTER CELLS BY VARIOUS CHEMICALS INCLUDING TUMOR PROMOTERS1980
- INHIBITION OF INTER-CELLULAR COMMUNICATION BY TUMOR-PROMOTING PHORBOL ESTERS1980
- Specific binding of phorbol ester tumor promotersProceedings of the National Academy of Sciences, 1980
- Tumor promoters inhibit metabolic cooperation in cocultures of epidermal and 3T3 cellsBiochemical and Biophysical Research Communications, 1979
- Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracerPublished by Elsevier ,1978
- Microinjection studies of duck globin messenger RNA translation in human and avian cellsCell, 1976