Isolation of new nonsense and frameshift mutants in the immunoglobulin ? heavy-chain gene of hybridoma cells

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Abstract
In order to expand the experimental material available for genetic and biochemical analyses of the natural immunoglobulin genes, we have isolated a variety of mutant mouse hybridoma cell lines. Some of these mutants have partial or complete deletions of the μ gene. Other mutants have nonsense or frameshift mutations in the exons encoding the variable and the second and third constant region domains of the μ heavy chain. When combined with earlier mutant data, this collection of genotypically and phenotypically tight mutants of known sequence spans most of the 10 kb of the μ gene, providing material for a variety of studies of genetic recombination and mRNA metabolism.