STAINING BACTERIAL SMEARS WITH FLUORESCENT ANTIBODY

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Abstract
Fluorescein-labeled antiglobulin was prepared against three major components of S. typhosa. Polyvalent fluorescein-labeled anti-globulin containing all three components was also prepared. When smears of the typhoid bacillus were exposed to the labeled antiglobulins, the three classes of antigens found in the cells could be stained specifically both collectively and individually with appropriate labeled anti-globulins. Six strains of S. typhosa, 1 Vi-containing paracolon, 1 S. Virginia, 1 S. derby, 5 S. typhimurium and 5 S. bredeney strains were examined by agglutination and fluorescent antibody reactions. The specificity of the staining established by inhibition and adsorption tests with fluorescent antibody agreed closely with that of agglutination tests for all serotypes. The procedure is offered as a tool potentially valuable for the identification of pathogenic bacteria in clinical specimens and should be of interest to clinicians and bacteriologists in public health and hospital laboratories.

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