Purification and characterization of equine infectious anemia virus
- 1 March 1976
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 51 (1-2) , 107-114
- https://doi.org/10.1007/bf01317839
Abstract
EIA virus was purified from equine fetal kidney cell cultures by PEG-precipitation, two sucrose-gradient sedimentations (5–30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20° C) of 1.162 and a sedimentation constant of S20W=656. Electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.This publication has 17 references indexed in Scilit:
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