MULTI-CSF-DEPENDENT COLONY FORMATION BY CELLS OF A MURINE HEMATOPOIETIC-CELL LINE - SPECIFICITY AND ACTION OF MULTI-CSF
- 1 January 1985
- journal article
- research article
- Vol. 65 (2) , 357-362
Abstract
Cells of the Multi-CSF [colony stimulating factor] (IL-3)-dependent hemopoietic cell line 32D c13 formed colonies of varying size in agar cultures stimulated by Multi-CSF. Colony formation was linear with respect to cultured cell numbers; colony numbers and size increased with increasing concentrations of Multi-CSF, and 32D colonies themselves contained a high frequency of clonogenic cells. Clonogenic 32D cells died in the absence of Multi-CSF (half-life 6 h), and most were unable to complete cell cycles in progress at the time of withdrawal of Multi-CSF. The concentration of Multi-CSF directly influenced the length of the cell cycle of dividing 32D cells. Purified GM [granulocyte-macrophage]-CSF, G [granulocyte]-CSF, or M-CSF [macrophage] had no capacity to support the survival or proliferation of 32D cells. Colonies formed by 32D cells appear to offer a useful model for analyzing the action of Multi-CSF in controlling self-renewal by clonogenic hemopoietic cells.This publication has 19 references indexed in Scilit:
- Identification in culture of a class of hemopoietic colony-forming units with extensive capability to self-renew and generate multipotential hemopoietic colonies.Proceedings of the National Academy of Sciences, 1982
- Establishment of continuous cultures of Thy1.2+, Lytl+,2−T cells with purified interleukin 3Cell, 1981
- The persisting (P) cell: histamine content, regulation by a T cell-derived factor, origin from a bone marrow precursor, and relationship to mast cells.Proceedings of the National Academy of Sciences, 1981
- Growth of factor-dependent hemopoietic precursor cell lines.The Journal of Experimental Medicine, 1980
- Clonal analysis of proliferation and differentiation of paired daughter cells: action of granulocyte-macrophage colony-stimulating factor on granulocyte-macrophage precursors.Proceedings of the National Academy of Sciences, 1980
- DIRECT STIMULATION BY PURIFIED GM-CSF OF THE PROLIFERATION OF MULTIPOTENTIAL AND ERYTHROID PRECURSOR CELLS1980
- Clonal analysis of the action of GM‐CSF on the proliferation and differentiation of myelomonocytic leukemic cellsInternational Journal of Cancer, 1979
- Colony formation in agar by multipotential hemopoietic cellsJournal of Cellular Physiology, 1979
- Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells.Journal of Biological Chemistry, 1977
- Purification and properties of colony-stimulating factor from mouse lung-conditioned medium.Journal of Biological Chemistry, 1977