St. Louis encephalitis virus temperature-sensitive mutants

Abstract
Summary Nine temperature-sensitive(ts) mutants of St. Louis encephalitis virus were isolated after “forced mutagenesis” with 5-fluorouracil or 5-azacytidine. Thets mutants could be grouped on the basis of RNA sythesis at 40° C, the nonpermissive temperature and complementation analysis. Four complementation groups were identified. Members of two of the groups were negative for RNA synthesis at 40° C while the remainder were positive.