Nitrogen Retention of Adult Human Subjects Fed Varying Quantities of Tryptophan

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Abstract
Young adult human subjects consumed 750, 625, 500, 375 and 250 mg of L-tryptophan per day in conjunction with quantities of other essential amino acids that exceeded twice the estimated minimal requirements. Essential amino acids were provided in part by cereals and in part by a mixture of L-isomers of crystalline amino acids. Glycine, glutamic acid and diammonium citrate were added to increase total dietary nitrogen to 6.0 g per day. Mean nitrogen balances were between 0.20 ± 0.38 and 0.57 ± 0.15 g per day, and did not differ significantly as a result of modifying tryptophan over a threefold range.