Synthesis of [Sar1, Val5, (4′‐Azido‐3′,5′‐ditritio)Phe8] Angiotensin II, a Photoaffinity Label for the Isolation of Angiotensin II Receptors Communication 1
- 9 July 1980
- journal article
- research article
- Published by Wiley in Helvetica Chimica Acta
- Vol. 63 (5) , 1308-1310
- https://doi.org/10.1002/hlca.19800630523
Abstract
The synthesis of [Sar1, Val5, (4′‐azido‐3′,5′‐ditritio)Phe8] angiotensin II from a iodinated precursor peptide is described. The principal problems of this synthesis and their resolution are discussed: (i) The β‐induced autophotolysis of the highly tritiated (73 Ci/mmol) and photosensitive label, and (ii) the absorption problems encountered during the purification of microgramm quantities. Such photoaffinity labels are being used for specific labeling and isolation of peptide hormone receptors.Keywords
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