Analysis of Self-Assembled Monolayer Interfaces by Electrospray Mass Spectrometry: A Gentle Approach
- 28 October 2003
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 75 (23) , 6741-6744
- https://doi.org/10.1021/ac0345897
Abstract
A general mass spectrometry technique for the characterization of alkanethiol-modified surfaces is presented. Alkanethiol self-assembled onto a gold surface (in this case, peptides were attached to the gold surface via a thiolate bond) was reductively desorbed in 0.05 M KOH in the presence of octadecyl-derivatized silica gel. The peptide adsorbed onto the silica gel, whereupon it could be filtered, washed to remove any salts, and then eluted using a mixture of 4:1 v/v methanol/water. The eluant containing the peptide was injected into a Fourier transform ion-cyclotron resonance mass spectrometer (FTICR/MS) via electrospray ionization. The spectrum showed no fragmentation of the peptide, demonstrating the gentleness of the technique. This simple procedure is not limited to FTICR/MS and could be adapted to other mass spectrometers.<br /Keywords
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