Identification of waterborne bacteria by the analysis of 16S-23S rRNA intergenic spacer region
Open Access
- 1 July 2004
- journal article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 97 (1) , 191-204
- https://doi.org/10.1111/j.1365-2672.2004.02288.x
Abstract
In this study, we evaluated, the use of universal primers, specific for the 16S-23S rRNA intergenic region, to detect and identify nine species that are of high interest for the microbiological control of water. The analysis of the fragments was carried out using a High Resolution acrylamide/bisacrylamide gels in a fluorescent automated DNA sequencer. The results showed specific profiles for each of the nine species but this technique failed to detect simultaneously micro-organisms in samples containing a mixed population. Nevertheless, the electrophoretic profiles obtained provided a very useful tool for the rapid and specific identification of water isolates. A possible new methodology for a rapid identification of pathogens in water.Keywords
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