The Effect of the Amount of Nucleic Acid Load on Hydroxylapatite Chromatography
- 1 March 1974
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 52 (3) , 267-271
- https://doi.org/10.1139/o74-041
Abstract
The effect of the nucleic acid to hydroxylapatite load ratio (μg/g) has been studied. It was found that as the load ratio was increased from 1 to 100 μg/g there was little change in the elution molarity of the nucleic acids whereas above 1000 μg/g the change could be substantial. Different hydroxylapatite preparations and different nucleic acids gave similar results but the load ratio dependence of the elution molarity was greater at 70 °C than at room temperature.Keywords
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