Direct role of the himA gene product in phage λ integration

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Abstract
The integration of phage .lambda. into the Escherichia coli chromosome is accomplished by a site-specific recombination between 2 unique DNA sequences (attB on the bacterial genome and attP on the phage) and requires proteins encoded by both the bacterium and the phage. Genetic and biochemical studies have shown that bacterial strains mutant in the himA gene, located at 38 min on the E. coli map, are defective in the activity of the host-encoded component. They are also defective for the growth of bacteriophage Mu, for precise excision of transposable antibiotic resistance determinants and for the synthesis of the .lambda. int gene product. The himA gene product (phimA), known to be a regulator of genes involved in integration, was shown to be 1 of 2 host polypeptides required for integrative recombination.

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