Exocrine Pancreas of Streptozotocin-Diabetes Rats Treated with Insulin

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Abstract
The effect of insulin treatment on the exocrine pancreas of streptozotocin-diabetes rats was investigated by light microscopy and EM. In diabetic rats treated with daily lente insulin injection for 4 wk, the islets became hyperplastic and proliferative, although degenerative and atrophic islets caused by streptozotocin remained if diabetic rats were not treated with insulin. Fibrosis and degeneration of the acinar cells were not found in all the diabetic rats by light microscopic examinations. EM examinations showed that acinar cells of the exocrine pancreas of the diabetic rats without insulin treatment were characterized by irregular dilatation and prominent lamellar arrangement of rough endoplasmic reticulum and by nuclear pyknosis. After 1 h of a single injection of regular insulin, rough endoplasmic reticulum of the acinar cells of the diabetic rats was rapidly activated, and many intracisternal granules appeared. When the daily injection of insulin was continued, the acinar cells showed regular arrangement of rough endoplasmic reticulum, much less vacuolarizations and less immature zymogen granules in comparison with those of the untreated diabetic rats. Exocrine pancreas of the insulin-treated rats revealed many autophagic vacuoles which were supposed to derive from lysosomes. Insulin probably had a repairing effect on the damaged acinar cells in the diabetic state.
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