Single beam optical tweezers setup with backscattered light detection for three-dimensional measurements on DNA and nanopores
- 1 June 2008
- journal article
- research article
- Published by AIP Publishing in Review of Scientific Instruments
- Vol. 79 (6) , 063702
- https://doi.org/10.1063/1.2938401
Abstract
We introduce a versatile and high precision three-dimensional optical tweezers setup with minimal optical interference to measure small forces and manipulate single molecules in the vicinity of a weak reflective surface. Our tweezers system integrates an inverted optical microscope with a single IR-laser beam that is spatially filtered in an appropriate way to allow force measurements in three dimensions with remarkably high precision when operated in backscattered light detection mode. The setup was tested by overstretching a λ -DNA in x and z directions (perpendicular and along the optical axis), and by manipulating individual λ -DNA molecules in the vicinity of a nanopore that allowed quantitative single molecule threading experiments with minimal optical interference.Keywords
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