Allogeneic hemopoietic stem cell transplantation using mouse spleen cells fractionated by lectins: in vitro study of cell fractions.

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Abstract
Mouse spleen cells sequentially agglutinated by soybean agglutinin (SBA) and peanut agglutinin (PNA) previously were shown to be sufficiently depleted of graft vs. host activity to allow reconstitution of lethally irradiated allogeneic recipient mice. Extent of T[thymus-derived]-cell depletion in this cell fraction by various in vitro assays, including cytotoxicity testing with anti-Thy-1 antiserum, mitogenic response to the T cell mitogens concanavalin A and phytohemagglutinin and allogeneic responsiveness in the mixed lymphocyte culture assay were tested. By these criteria the SBA+, PNA+ spleen fraction, used previously in the in vivo experiments, possessed about 1% T cell contamination. The slight contamination with T cells previously found in the singly agglutinated SBA fraction can be removed by a 2nd fractionation with SBA, eliminating the possibility that a minor T cell subpopulation bears receptors for SBA. The twice-agglutinated fraction, by SBA and PNA or by SBA alone, contains a significant number of prothymocytes, indicating that mouse prothymocytes bear receptors for both SBA and PNA. The implication of these findings to bone marrow transplantation in humans was discussed.