Characterization of mutants involving partial exon duplications in thehprt gene of Chinese hamster V79 cells
- 1 May 1996
- journal article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 22 (3) , 201-210
- https://doi.org/10.1007/bf02369910
Abstract
Sequencing ofhprt cDNA revealed that three spontaneous mutants in V79 Chinese hamster cells exhibit tandem duplications of exon(s), i.e., either exons 2 and 3 or exon 7. Sequences of different sizes (4.5—8 Kb) were found to be duplicated and inserted in tandem into thehprt gene. These mutants demonstrated spontaneous reversion frequencies which were about 40-fold higher than those observed with other types of spontaneous mutants, but on the same order of magnitude as spontaneous reversions in Sp5, a mutant with a duplication insertion involving exon 2 in this gene. These data suggest that all of the duplications found have the same genetic instability, regardless of the type, size or position of the duplictaed fragment. The coding sequence of thehprt cDNA and the restriction pattern of the revertants were virtually identical to the wild-type, indicating restoration of a functionalhprt gene by precise deletion of the duplicated fragment.Keywords
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