In situPYRIMIDINE DIMER DETERMINATION BY LASER CYTOMETRY
- 1 April 1989
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 49 (4) , 523-526
- https://doi.org/10.1111/j.1751-1097.1989.tb09205.x
Abstract
Abstract—By using antiserum against pyrimidine dimers and argon‐laser imaging microspectrofluoro‐metry, we established a new method to determine UV‐induced pyrimidine dimers and their repair in individual human cells. The method was sensitive enough to determine dimers induced by UV dose as low as 2 J/m2. Normal human cells repaired 50 and 60% of total damage within 8 and 24 h after UV irradiation (20 J/m2), butXeroderma pigmentosumcells (complementation group A) were unable to repair any within the same period. Therefore, the method proved to be a quick, easy, sensitive and accurate means to determine pyrimidine dimersin situ.This publication has 26 references indexed in Scilit:
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