Location, structure, and function of the target of a transcriptional activator protein.
Open Access
- 15 December 1994
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 8 (24) , 3058-3067
- https://doi.org/10.1101/gad.8.24.3058
Abstract
We have isolated and characterized single-amino-acid substitution mutants of RNA polymerase alpha subunit defective in CAP-dependent transcription at the lac promoter but not defective in CAP-independent transcription. Our results establish that (1) amino acids 258-265 of alpha constitute an "activation target" essential for CAP-dependent transcription at the lac promoter but not essential for CAP-independent transcription, (2) amino acid 261 is the most critical amino acid of the activation target, (3) amino acid 261 is distinct from the determinants for alpha-DNA interaction, and (4) the activation target may fold as a surface amphipathic alpha-helix. We propose a model for transcriptional activation at the lac promoter that integrates these and other recent results regarding transcriptional activation and RNA polymerase structure and function.Keywords
This publication has 47 references indexed in Scilit:
- Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP) I. Saturation and alanine-scanning mutagenesisJournal of Molecular Biology, 1994
- Drosophila TAF II 150: Similarity to Yeast Gene TSM-1 and Specific Binding to Core Promoter DNAScience, 1994
- Factor Independent Activation of rrnB P1Journal of Molecular Biology, 1994
- Comparison of a Structural and a Functional EpitopeJournal of Molecular Biology, 1993
- Prediction of Protein Secondary Structure at Better than 70% AccuracyJournal of Molecular Biology, 1993
- Mapping the cAMP receptor protein contact site on the α subunit of Escherichia coli RNA polymeraseMolecular Microbiology, 1992
- High resolution functional analysis of antibody-antigen interactionsJournal of Molecular Biology, 1992
- [6] Efficient site-directed mutagenesis using uracil-containing DNAPublished by Elsevier ,1991
- Nucleotide sequence changes produced by mutations in the lac promoter of Escherichia coliJournal of Molecular Biology, 1977
- The nature of the accessible and buried surfaces in proteinsJournal of Molecular Biology, 1976