An Escherichia coli ribonuclease which removes an extra nucleotide from a biosynthetic intermediate of bacteriophage T4 proline transfer RNA
Open Access
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (11) , 4129-4140
- https://doi.org/10.1093/nar/5.11.4129
Abstract
The biosynthesis of bacteriophage T4 tRNAPro, tRNASer and tRNAI1e requires enzymstic removal of extra nucleotides from the 3′ terminus of the respective precursor RNAs. A ribonuclease activity capable of catalyzing such reactions has been partially purified from uninfected Escherichia coli using an artificial precursor RNA as substrate. A number of ribonuclease activities were resolved during purification. Use of E. coli strain BN, a mutant known to be deficient in the relevant ribonuclease activity, permitted us to identify it in wild-type cells. This activity was designated the BN ribonuclease. BN ribonuclease had an apparent molecular weight of 35,000 as measured by Sephadex gel filtration. Mg2+ was required for activity, which was optimal at of [Mg2+] of 2mM. Activity did not require monovalent cations K+ or Na+. BN ribonuclease was less efficient at removing extra residues in the biosynthesis of tRNASer and RNAI1e than in the biosynthesis of RNAPro.Keywords
This publication has 11 references indexed in Scilit:
- Evolution of the biosynthesis of 3′-terminal C-C-A residues in T-even bacteriophage transfer RNAsJournal of Molecular Biology, 1978
- Identification of an Escherichia coli nuclease acting on structurally altered transfer RNA moleculesJournal of Biological Chemistry, 1978
- Transfer ribonucleic acid biosynthesis. Substrate specificity of ribonuclease P.Journal of Biological Chemistry, 1976
- Five steps in the conversion of a large precursor RNA into bacteriophage proline and serine transfer RNAsJournal of Molecular Biology, 1975
- A novel function of Escherichia coli transfer RNA nucleotidyltransferase. Biosynthesis of the C-C-A sequence in a phage T4 transfer RNA precursor.Journal of Biological Chemistry, 1975
- A mutant of escherichia coli defective in removing 3′ terminal nucleotides from some transfer RNA precursor moleculesCell, 1975
- In vitro synthesis of transfer RNA. II. Identification of required enzymatic activitiesJournal of Biological Chemistry, 1975
- The nucleotide sequence of the dimeric precursor to glutamine and leucine transfer RNAs coded by bacteriophage T4Journal of Molecular Biology, 1975
- Study of the Escherichia coli tRNA nucleotidyltransferase. Specificity of the enzyme for nucleoside triphosphatesBiochimie, 1975
- Isolation of an Escherichia coli strain restricting bacteriophage suppressorMolecular Genetics and Genomics, 1973