OXIDASE POSITIVE RODS FROM CASES OF SUSPECTED GONORRHEA - COMPARISON OF CONVENTIONAL, GAS-CHROMATOGRAPHIC AND GENETIC METHODS OF IDENTIFICATION

  • 1 January 1977
    • journal article
    • research article
    • Vol. 85  (1) , 27-37
Abstract
Genito-urethral specimens from 3260 women and 1170 men, with symptoms suggestive of gonorrhea, were examined for growth of oxidase positive rod-shaped bacteria and of gonococci. Moraxella osloensis was identified in 26 cases (0.64% of women and 0.43% of men). Three patients harbored phenylalanine negative (or weakly reacting) and tryptophan deaminase negative M. phenylpyruvica and, in 3 cases, a Flavobacterium sp. was detected. Among 6 oropharyngeal specimens from patients suspected of gonorrhoea, 2 yielded growth of oxidase positive rods, M. kingii and Neisseria elongata, respectively. N. gonorrhoeae was isolated from 537 patients, i.e., 12.1% of all cases. The isolates of oxidase positive rods were in most cases completely identified by streptomycin resistance transformation. On this basis, the diagnostic reliability of some morphological and cultural-biochemical tests and gas chromatography was examined. Gas chromatographic analysis of fatty acid and alcohol composition of whole cells proved distinctive of species defined genetically, irrespective of confusing behavior of some strains in other tests.

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