A METHOD FOR ELECTRON-MICROSCOPIC PREPARATION OF CULTURED-CELLS (MONOLAYER) IN A NEW TEST CHAMBER (TCSC-1)

  • 1 January 1981
    • journal article
    • research article
    • Vol. 84  (2) , 117-120
Abstract
A time-saving method is described for the preparation of cultivated cells (monolayer) for EM using a new test chamber system (TCSC-1). The versatile chamber is fitted with a screw-in PTFE-adapter which connects via a silicone ring gasket to the surface on which the cells are grown. All procedures (fixation, staining, embedding) were performed directly in the chamber. The cells [epithelial liver cells] were grown as a monolayer on polyester foils. After embedding of the cells, the foils can be easily taken off. Removal of the foil does not in any way effect the quality of the 1st slice for EM examination. The resin block can also be easily pressed out from the PTFE adapter.