Abstract
A comparative study on the electrophoretic patterns of proteins from rye protoplasts has been carried out using a two-dimensional electrophoretic gel system. Rye was cold hardened by cold acclimation or desiccation stress. The two treatments did not induce similar protein pattern changes indicating different metabolic and physiological responses of the cell to these two stresses. A protein band with an apparent molecular weight of 46 000 increased upon hardening by both treatments and was correlated to freezing tolerance. Proteins in this band may play a role in the expression of freezing tolerance of the cell.

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