Increased Mutation Rate from Aged Datura Pollen

Abstract
Pollen from Datura plants of the standard line, stored air-dry at 30[degree] C, gave some successful pollinations at intervals up to 13 days. In 193 plants, derived from pollinations with aged pollen, 29 pollen abortion mutations were found. Of these, 18 were of the chromosomal and 11 were of the gene type. No mutations were found in 191 control plants. For the larger progenies the rate of pollen mutations was 13.9% from 8- to 10-day-old pollen, and 5.7% from 5- to 6-day-old pollen. In the smaller progenies, from pollen aged 4-13 days, there were 9 mutations among 18 plants. It is suggested that the method of aging pollen may be of wide applicability for inducing mutations.

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