Separation between modulator-dependent protein kinases I and II
- 1 March 1981
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 37 (3) , 235-237
- https://doi.org/10.1007/bf01991628
Abstract
The separation of modulator-dependent protein kinase I from modulator-dependent protein kinase II obtained from the lungs of sexually premature male mice was accomplished by Sephadex G-200 gel filtration. After preincubation of a mouse lung cytosol fraction with arginine-rich histone, theophylline, cyclic GMP and crude protein kinase modulator, a cyclic GMP-dependent protein kinase activity peak present in a non-preincubated sample completely disappeared and was replaced by a late-eluted modulator-dependent protein kinase II peak. There was a difference in substrate specificity between modulator-dependent protein kinase I and modulator-dependent protein kinase II despite their similar dependence on crude protein kinase modulator or partially purified stimulatory protein kinase modulator for their maximal activities.This publication has 10 references indexed in Scilit:
- Separation of modulator-dependent protein kinase (type I) from cyclic GMP-dependent protein kinase in mouse testesCellular and Molecular Life Sciences, 1980
- Conversion of mammalian cyclic GMP-dependent protein kinase into modulator-dependent protein kinase (type II) in vitroCellular and Molecular Life Sciences, 1980
- Molecular properties and mode of action of homogeneous preparation of stimulatory modulator of cyclic GMP-dependent protein kinase from the heart.Journal of Biological Chemistry, 1978
- Studies on the cyclic GMP-dependent protein kinase from bovine aorta. Possible existence of a catalytic subunit.Journal of Biological Chemistry, 1977
- Guanosine cyclic monophosphate-dependent protein kinase from foetal calf heart. Purification, general properties and catalytic subunitBiochemical Journal, 1977
- Stimulatory modulator of guanosine 3′:5′-monophosphate-dependent protein kinase from mammalian tissuesBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- Purification and general properties of guanosine 3':5'-monophosphate-dependent protein kinase from guinea pig fetal lung.Journal of Biological Chemistry, 1976
- Guanosine 3′:5′-Monophosphate-Dependent Protein Kinases in Mammalian TissuesProceedings of the National Academy of Sciences, 1974
- Krebs EG: Purification and characterization of a protein inhibitor of adenosine 3',5'-monophosphate-dependent protein kinases.1971
- Factors affecting the activity of muscle glycogen synthetaseArchives of Biochemistry and Biophysics, 1966