Rapid Separation of Fat for Pesticide Residue Analysis of Milk Products

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Abstract
Other than ether extraction of the fat, current procedures, such as Babcock test fat or alkaline digestion of the milk, give incomplete recovery, alter, or destroy some pesticide residues in milk fat. A reagent which gives as good pesticide residue recovery as ether extraction consists of 25 g sodium tetraphosphate, 12 ml Triton X-100 (Rohm and Haas Co.), 25 g urea and 50 ml isopropyl alc in 500 ml water. Add 25 ml of reagent to 25 ml of fluid milk product, or cheese emulsified with the reagent, heat for 10 min in an 18 gm, 50% Babcock cream test bottle or a 50 ml volumetric flask, in a boiling water bath. Add hot water, centrifuge or hold in warm water bath to permit clear fat layer to rise. Recovery data on 10 pesticides is given.