In-cell matrix-assisted laser desorption-ionization fourier transform ion cyclotron resonance mass spectrometry
- 1 October 1996
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Society for Mass Spectrometry
- Vol. 7 (10) , 1026-1033
- https://doi.org/10.1016/1044-0305(96)00029-3
Abstract
A new internal matrix-assisted laser desorption-ionization (MALDI) Fourier transform ion cyclotron resonance-mass spectrometry (FTICR-MS) method is introduced. The target is directly positioned at one trapping electrode of a single cylindrical ion cyclotron resonance (ICR) cell and becomes a part of it. The ionization occurs inside the ICR cell in contrast to external or near-cell MALDI-FTICR-MS techniques. Very efficient trapping and mass resolving power better than unit resolution of singly charged peptides and proteins ions up to 2000 u is possible by using only basic FTICR-MS techniques. The sole application of a pulsed retarding potential increases the mass range to 6000 u. No collisional cooling and quadrupolar excitation was done. Sensitivities below 1 fmol, and ion storage times of more than 15 s are shown. High resolving powers of 16,000 and 56,000 are obtained on bovine insulin (5.7 ku) and gramicidin D (1.9 ku), respectively.Keywords
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