Insulin-Sepharose and the Dynamics of Insulin Action

Abstract
In terms of the rate of accumulation of α-aminoisobutyric acid, mammary epithelial cells from mature virgin mice respond rapidly to insulin covalently bound to sepharose particles, but do not respond initially to soluble insulin in vitro. The response to insulin-sepharose is prevented if the cells are first exposed to insulin. These observations may provide new insight into the dynamics of insulin action.