GENETIC CONTROL OF COMBINING SITES OF INSULIN ANTIBODIES PRODUCED BY GUINEA PIGS

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Abstract
These experiments were designed to test whether genetic factors control the configuration of the combining sites of insulin antibodies from inbred str 2, str 13 guinea pigs, the F1 (str 2 x str 13) and F2 (F1 x F1) hybrids. Differences in configuration of the insulin antibody combining sites were discerned by measuring the percentage of antibodies in a test antiserum (ABt) which bound to the exposed insulin determinants on an insoluble insulin complex (IC) which had been saturated with antibodies from a different antiserum (ABs). No configurational differences of antibody combining sites were noted in guinea pigs of the same strain. Configurational differences in insulin antibody combining sites were noted between str 2 and str 13 guinea pigs. Segregation of differences in antibody combining site configurations was noted in F1 and F2 guinea pigs. It is therefore, quite probable that genetic factors control the configuration of insulin antibody combining sites in guinea pigs. Furthermore, insulin antibody combining sites from F2 animals can have configurations that are different than the configurations of insulin antibodies noted in either of the two inbred parental strains. It is therefore also possible that the configurations of the combining sites of insulin antibodies produced in guinea pigs is controlled by more than one gene and not by multiple alleles at a given gene locus.